Skip to main content

Those geneticists and their Excel

Mistaken Identifiers: Gene name errors can be introduced inadvertently when using Excel in bioinformatics

If you are too lazy to get to the punchline:

"MatchMiner [1] and GoMiner [2] are two bioinformatics program packages we
published recently in another Biomed Central Journal, Genome Biology. When we
were beta-testing those programs on microarray data, a frustrating problem
occurred repeatedly: Some gene names kept bouncing back as "unknown." A little
detective work revealed the reason: Use of one of the research community's most
valuable and extensively applied tools for manipulation of genomic data can
introduce erroneous names. A default date conversion feature in Excel (Microsoft
Corp., Redmond, WA) was altering gene names that it considered to look like
dates. For example, the tumor suppressor DEC1 [Deleted in Esophageal Cancer 1]
[3] was being converted to '1-DEC.' Figure 1 lists 30 gene names that suffer an
analogous fate."
Labels:

Comments

Post a Comment

Popular posts from this blog

A note on Python's __exit__() and errors

Python's context managers are a very neat way of handling code that needs a teardown once you are done. Python objects have do have a destructor method ( __del__ ) called right before the last instance of the object is about to be destroyed. You can do a teardown there. However there is a lot of fine print to the __del__ method. A cleaner way of doing tear-downs is through Python's context manager , manifested as the with keyword. class CrushMe: def __init__(self): self.f = open('test.txt', 'w') def foo(self, a, b): self.f.write(str(a - b)) def __enter__(self): return self def __exit__(self, exc_type, exc_val, exc_tb): self.f.close() return True with CrushMe() as c: c.foo(2, 3) One thing that is important, and that got me just now, is error handling. I made the mistake of ignoring all those 'junk' arguments ( exc_type, exc_val, exc_tb ). I just skimmed the docs and what popped out is that you need to return True or...
Post a Comment
Read more

Store numpy arrays in sqlite

Use numpy.getbuffer (or sqlite3.Binary ) in combination with numpy.frombuffer to lug numpy data in and out of the sqlite3 database: import sqlite3, numpy r1d = numpy.random.randn(10) con = sqlite3.connect(':memory:') con.execute("CREATE TABLE eye(id INTEGER PRIMARY KEY, desc TEXT, data BLOB)") con.execute("INSERT INTO eye(desc,data) VALUES(?,?)", ("1d", sqlite3.Binary(r1d))) con.execute("INSERT INTO eye(desc,data) VALUES(?,?)", ("1d", numpy.getbuffer(r1d))) res = con.execute("SELECT * FROM eye").fetchall() con.close() #res -> #[(1, u'1d', <read-write buffer ptr 0x10371b220, size 80 at 0x10371b1e0>), # (2, u'1d', <read-write buffer ptr 0x10371b190, size 80 at 0x10371b150>)] print r1d - numpy.frombuffer(res[0][2]) #->[ 0. 0. 0. 0. 0. 0. 0. 0. 0. 0.] print r1d - numpy.frombuffer(res[1][2]) #->[ 0. 0. 0. 0. 0. 0. 0. 0. 0. 0.] Note that for work where data ty...
Post a Comment
Read more